Coding

Part:BBa_K2881007:Design

Designed by: Zhijian Li   Group: iGEM18_UC_San_Diego   (2018-10-10)


mMBD-eGFP-avi-His (XbaI)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 220
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1009
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. thrombin site is inserted at the end in case His6 interfere with specific uses. 2. sequence has been codon optimized for BL-21 expression with IDT software. 3. Assembly of this part strictly followed the protocol provided on https://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones; As a result, XbaI and SpeI no longer exist in the vector. As a result, even this part contains a XbaI site in the middle, it should not be interfered when EcoRI and SpeI are used to obtain the part from the vector



Source

gblock fragments were purchased from IDT DNA.

References